RESUMO
Greenhouse cover pollution with green shading composed of dust, microalgae and bacteria is a severe problem in tropical areas. The shading results in lower greenhouse indoor light intensity reducing the yield and quality of protected horticulture crops. However, few studies have focused on environmentally efficient ways to remove green shading to increase greenhouse production. In this study, five purified microalgae were isolated from the green shading of three greenhouse roofs and were identified using morphological and molecular assessments. The effects of Ultraviolet-C irradiation (UV-C, 254 nm) at doses of 100, 200 and 300 mJ cm-2 on the growth of GLY-1 microalgae were investigated. The results indicated that five purified microalgae all appeared to belong to the genus of Jaagichlorella. The purified microalgae cell density and chlorophyll content decreased respectively by 26.89-74.44 % and 42.02-77.31 % at 1-3 d after UV-C treatment with doses ranging from 100 to 300 mJ cm-2. The inhibition of the growth rate of microalgae was significantly positively correlated with the UV-C irradiation dose and significantly negatively correlated with treatment time. In summary, UV-C irradiation treatment at 300 mJ cm-2 and 3 d could substantially inhibit microalgae growth in green shading on greenhouse covers. UV-C irradiation could be an effective method for solving the problem of greenhouse cover pollution with microalgae.
